PRINCIPLE
Proteins are large molecules composed of covalently linked amino acids. Depending on electron distributions resulting from covalent or ionic bonding of structural sub-groups,proteins can be either polar or nonpolar at a given pH. In the Helena TITAN GEL Serum Protein procedure,proteins are separated according to their re-spective electrical charges at8.4-8.8 on agarose gel us-ing both the electrophoretic and electroendsmotic forces present in the system. The proteins are then stained with Amido Black staining solution.
REAGENT
1.TITAN：Each plate contains agarose in barbital buffer with 0.02% thimerosal added as a preservative.
WARNING：FOR IN-VITRO DIAGNOSTIC USE ONLY.
Preparation for Use：The plates are ready for use as packaged
Storage and Stability：The plates should be stored at room temperature (15 to 30度C) and are stable until the expiration date indicated on the package. The plates must be stored in the protective packaging in which they are shipped.DO NOT REFRIGERATE OR FREEZE THE PLATES.
Signs of Deterioration：Any of the following condi-tions may indicate deterioration of the plate：(1)crys-talline appearance indicating the agarose has been frozen,(2)cracking and peeling indicating drying of the agarose,(3)bacterial growth indicating contamin-tion.
[ 本帖最後由 robert1984 於 2007-12-1 22:27 編輯 ] |
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