This experiment the tea variety which is in sole possession of using
Taiwan, screens the high content the son caffeine and the ester
caffeine, and establishes the Taiwan tea the son caffeine content data
material, provides the tea to change the field breeding as well as the
development tea health foods basis.
This experiment develops the HPLC analysis method may simultaneously
the quota 7 kind of teas main sons caffeine, the analysis material
including 20 kind of teas series TA01~20 number, partial fermentation
tea 14 kind, the wild 12 kinds and so on total have 52 with
the variety 5 kinds to grow tea plants carries on the
quantitative analysis. How much is the result discovery caffeine
content in order Epigallocatechin gallate (EGCG) Epigallocatechin
(EGC) Epicatechin (EC) Gallocatechin (GC) Epicatechin gallate (ECG)
Catechin (C).The ester caffeine is highest by the EGCG content, in each hectogram
dry leaf includes 7.25±2.67 (g/100g d.w.) The C drifting away
caffeine is highest by the EGC content, for 2.59±1.02 (g/100g d.w.)
The C always caffeine (TC=GC+EGC+C+EC+EGCG+ECG) the highest content is
the TM03 strain 16.69±0.12 (g/100g d.w.) The A lowest content is the
TC04 strain 7.91±0.26 (g/100g d.w.) above a t time.
In 7 kind of individual caffeine content, GC, EC and the ECG variation
bigger (CV is bigger than 50%), other 4 kinds relative are smaller.
TA03, TA07, TA08, TA12, TA14, TA17, TA18, TM03 and TM05 these variety
tea tree's TC perhaps TCG all have a higher content, based on the
variety resources protection, or will develop the health foods in
accordance to the future the demand, all will continue forever the
retention.
The methylation son caffeine (EGCG3 "Me) has anti- allergic the
effect, therefore this research in view of specific tea tree leaf
blade further line of EGCG3" the Me content relativity analysis,
finally demonstrated analyzes in 50 kind of teas, EGCG3 "the Me
content is higher than 0.6% dry Ye Zhong to have 16 kind of tea trees,
is bigger than 0.8% has 14 kinds, is bigger than 1% has 9 kinds, only
occupies completely investigates sample 1/3; Other 34 kind of teas
relative content is quite low. Thus it may be known, the different tea
tree originates different tea EGCG3 "Me content difference very large,
it is necessary further to carry on large-scale screening to the
existing Taiwan tea tree to have higher content EGCG3" the Me tea
tree.
This experiment also chose 40 kind of Taiwan tea to copy the sector to
its nucleus in vivo (Internal Transcribed Spacer, ITS) the
sequence decides the foreword, finally discovered, in distributed to
Taiwan kind or variety nrDNA area sequence their sequence
length between 594 □647, however with mainland China the
sequence length probably between 476 496, also various sequences
similar in a tea between was 0.571~1, with mainland tea similar
between 0.741~0.946, this result not only has demonstrated the tea
seedling source multiplicity, Simultaneously also provided has
further decided the possibility by DNA which a tea, the mainland tea
habitat originated.
This research HPLC of fingerprint atlas information bank achievement
establishment tea active constituent, and provides the platform
technology which the minute original decides, not only
promotes a tea the domestic and foreign markets competitive ability,
simultaneously also promotes the tea fresh technique industry to
continue forever the development. |
